Immunohistochemical analysis on cortex-to-cortex healing after mandibular vertical ramus osteotomy: A preliminary study

Publication date: Available online 13 October 2017
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Hwi-Dong Jung, Sang Yoon Kim, Han-Sung Jung, Hyung-Sik Park, Young-Soo Jung
PurposeThis study aimed to analyze the expression of specific cytokines among TGF-β superfamily over postoperative periods after mandibular VRO.Materials and MethodsA total of 4 beagle dogs were enrolled and euthanized at 1, 2, 4, and 8 weeks postoperatively for immunohistochemical (IHC) analysis using 6 specific antibodies (BMP-2/4, BMP-7, TGF-ß2, TGF-β3, MMP-3, and VEGF). The results from the surgical site and control (adjacent area) were compared.ResultsGeneralized upregulation of BMP-2/4 was observed in all healing periods while the strongest expression of BMP-7 was observed at 1 week. The strongest expression of TGF-ß2 was observed at 8 weeks with increasing pattern. The strong expression of TGF-β3 was observed at 1 and 4 weeks while VEGF at 1 week with decreasing pattern. There were no notable uptakes detected with 6 specific antobodies on adjacent bone (control).ConclusionThe absence of internal fixation following VRO lead to dynamic healing with a specific expression pattern of BMP-7 and TGF-β2. The anatomical factors including sufficient preexisting vascularity lead to the earlier expression pattern of VEGF.



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