Guided bone regeneration in calvarial critical size bony defect using a double-layer resorbable collagen membrane covering a xenograft: a histological and histomorphometric study in rats

Abstract

Purpose

The aim of the present study was to evaluate histologically and histomorphometrically the bone regeneration in critical size calvarial defects in rats grafted with either a deproteinized bovine bone mineral (DBBM) alone or in combination with a single or double layer of native bilayer collagen membrane (NBCM). The secondary objective was to evaluate histologically and histomorphometrically the residual DBBM in these defects.

Material and methods

Thirty-two Wistar rats were divided into two groups: a control group of 16 rats with two critical size calvarial defects (CSD) of 5 mm performed each on either side of the median sagittal suture, where the frontal defect remained without any filling (negative control), while the occipital defect (positive control) was filled with DBBM; and then a test group of 16 rats, with two CSD filled with DBBM and covered by either a single (SM) or a double layer (DM) of NBCM. The animals were sacrificed at 4 and 8 weeks.

Results

At 1 month, the histological and histomorphometric analysis showed new bone formation (NBF) in the defects that received only DBBM, DBBM+DM, and DBBM+SM (11.5, 17.3, and 22.7%, respectively), while the negative control defects showed only 0.4% of new bone formation. At 2 months, the histological and histomorphometric analysis showed NBF in the defects that received only DBBM, DBBM+DM, and DBBM+SM (16.8, 24.5, and 37%, respectively), while the negative control defects showed only 0.9% of new bone formation. The residual xenogeneic material (RXM) was higher in defects covered by SM (30.2% at 1 month and 25.3% at 2 months) or DM (32.5% at 1 month and 28.5% at 2 months) compared with defects that were not covered by membranes (15.3% at 1 month and 9.4% at 2 months).

Conclusions

This study demonstrated that GBR with a xenogeneic material in rat calvarial (CSD) of 5 mm requires the application of resorbable collagen membranes in either single or double layer, and a single layer alone is sufficient to promote this regeneration.

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