Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Τετάρτη 5 Σεπτεμβρίου 2018

Protein expression in submandibular glands of young rats is modified by a high-fat/high-sugar maternal diet

Publication date: Available online 5 September 2018

Source: Archives of Oral Biology

Author(s): Martine Morzel, Hélène Brignot, Franck Ménétrier, Géraldine Lucchi, Vincent Paillé, Patricia Parnet, Sophie Nicklaus, Marie-Chantal Canivenc-Lavier

Abstract
Objective

Maternal diet has consequences on many organs of the offspring, but salivary glands have received little attention despite the importance of the saliva secretory function in oral health and control of food intake. The objective of this work was therefore to document in rats the impact of maternal high-fat / high-sugar diet (Western Diet) on submandibular glands of the progeny.

Design

Sprague-Dawley rat dams were fed either a Western diet or control diet during gestation and lactation and their pups were sacrificed 25 days after birth. The pups' submandibular gland protein content was characterized by means of 2D-electrophoresis followed by LC-MS/MS. Data were further analyzed by Gene Ontology enrichment analysis and protein-protein interactions mapping. The expression of two specific proteins was also evaluated using immunohistochemistry.

Results

Combining both male and female pups (n = 18), proteome analysis revealed that proteins involved in protein quality control (e.g. heat shock proteins, proteasome sub-units) and microtubule proteins were over-expressed in Western diet conditions, which may translate intense metabolic activity. A cluster of proteins controlling oxidative stress (e.g. Glutathione peroxidases, peroxiredoxin) and enhancement of the antioxidant activity molecular function were also characteristic of maternal Western diet as well as under-expression of annexin A5. The down-regulating effect of maternal Western diet on Annexin A5 expression was significant only for males (p < 0.05).

Conclusions

A maternal Western diet modifies the protein composition of the offspring's salivary glands, which may have consequences on the salivary function.



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