The genomic landscape of UM-SCC oral cavity squamous cell carcinoma cell lines

Publication date: December 2018

Source: Oral Oncology, Volume 87

Author(s): Megan L. Ludwig, Aditi Kulkarni, Andrew C. Birkeland, Nicole L. Michmerhuizen, Susan K. Foltin, Jacqueline E. Mann, Rebecca C. Hoesli, Samantha N. Devenport, Brittany M. Jewell, Andrew G. Shuman, Matthew E. Spector, Thomas E. Carey, Hui Jiang, J. Chad Brenner

Abstract

Objectives

We sought to describe the genetic complexity of 14 UM-SCC oral cavity cancer cell lines that have remained uncharacterized despite being used as model systems for decades.

Materials and Methods

We performed exome sequencing on 14 oral cavity UM-SCC cell lines and denote the mutational profile of each line. We used a SNP array to profile the multiple copy number variations of each cell line and use immunoblotting to compare alterations to protein expression of commonly amplified genes (EGFR, PIK3CA, etc.). RNA sequencing was performed to characterize the expression of genes with copy number alterations.

Results

The cell lines displayed a highly complex network of genetic aberrations that was consistent with alterations identified in the HNSCC TCGA project including PIK3CA amplification, CDKN2A deletion, as well as TP53 and CASP8 mutations, enabling genetic stratification of each cell line in the panel. Copy number FISH and spectral karyotyping analysis demonstrate that cell lines retain chromosomal heterogeneity.

Conclusions

Collectively, we developed an important resource for future oral cavity HNSCC cell line studies and highlight the complexity of genomic aberrations in cell lines.

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