Comparison of FASN and COX-2 immunoexpression between embryonal, benign and malignant odontogenic tissues

Publication date: Available online 31 December 2018

Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Author(s): Celeste Sánchez-Romero, Adalberto Mosqueda-Taylor, Wilson Delgado-Azañero, Oslei Paes de Almeida, Ronell Bologna-Molina

ABSTRACT

Objectives

To analyze the immunohistochemical expression of fatty acid synthase (FASN) and cyclooxygenase-2 (COX‑2) in tooth germ (TG), ameloblastoma (AM), ameloblastic carcinoma (AC), ameloblastic fibroma (AF) and ameloblastic fibrosarcoma (AFS).

Study design

Immunohistochemistry for FASN and COX-2 was performed in 10 TG, 44 AM, 10 AC, 9 AF and 5 AFS specimens. The results were analyzed using the immunoreactive score (IRS) and Kruskal-Wallis test followed by Dunn's post-test.

Results

Most TG were strongly positive for FASN, while COX-2 was weak or negative. All AM and AC expressed both proteins. In AF, FASN and COX-2 were variably expressed in the epithelium and negative in the mesenchyme. In AFS, FASN was strongly positive in the malignant mesenchyme and variable in the epithelium; COX-2 was focal or weak in both components. FASN showed significant differences in the following comparisons: TG vs AC, AM vs AC, and AF vs AFS. Differences in COX-2 were significant when comparing TG with AM, AC and AF.

Conclusion

The results suggest that FASN and COX-2 overexpression may have a role in the pathogenesis of AM and AC¸ while in AFS, only FASN seems to be involved. Further studies are necessary to clarify these mechanisms and their clinical implications.

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