Application of denaturing gradient gel electrophoresis to the analysis of bacterial communities associated with asymptomatic and symptomatic pericoronitis

Publication date: Available online 18 August 2017
Source:Journal of Oral and Maxillofacial Surgery
Author(s): Xin Zhang, Zheng Sun, Qiubo Yang
ObjectiveDenaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial communities associated with asymptomatic and symptomatic pericoronitis. The aim of the study was to compare fingerprinting patterns of these two clinical conditions.MethodsThe microbiota of mandibular third molar pockets associated with asymptomatic or symptomatic pericoronitis cases were collected and was profiled by the polymerase chain reaction (PCR)-DGGE method. Banding patterns were then compared by cluster analyses techniques.Results13 symptomatic pericoronitis and 7 asymptomatic pericoronitis samples were collected. Comparative analysis of the two clinical conditions revealed bands that were common to both symptomatic and asymptomatic cases, but most DGGE bands appeared to be unique to the clinical condition. No single band occurred in all profiles. The mean number of bands detected in the 16S rDNA community profiles was 23.8 ± 4.2 (range 19–34) for samples from symptomatic cases and 24.1 ± 2.4 (range 21–29) for those from asymptomatic cases. A cluster analysis and Multi-Dimensional Scaling analysis of DGGE banding pattern showed a distinction in the similarity of banding patterns according to the presence or absence of symptoms.ConclusionsThese results suggest that the diversity of pericoronal pocket microbiota in asymptomatic pericoronitis cases are significantly different from that of symptomatic cases.



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