Evaluation of different therapeutic Carnoy's formulations on hard human tissues: A Raman microspectroscopy, microhardness, and scanning electron microscopy study

Publication date: Available online 21 February 2018
Source:Journal of Cranio-Maxillofacial Surgery
Author(s): Francisco Samuel Rodrigues Carvalho, Victor Pinheiro Feitosa, Paulo Goberlânio de Barros Silva, Eduardo Costa Studart Soares, Thyciana Rodrigues Ribeiro, Cristiane Sá Roriz Fonteles, Fábio Wildson Gurgel Costa
PurposeTo evaluate different therapeutic Carnoy's solution formulations on hard human tissues.Materials and MethodsAn in vitro study was performed with human teeth (n=36) and bone fragments (n=18), randomly divided into two experimental groups (Group I = Carnoy solution with chloroform; Group II = Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing. Raman microspectroscopy, Knoop microhardness test, and scanning electron microscopy with energy dispersive X-ray spectroscopy were used.ResultsThere was demineralization of dental structures regarding mineral/matrix and carbonate/phosphate ratios (GI versus GII, p < 0.05). The presence of chloroform resulted in a statistically significant reduction of the teeth surface microhardness (p = 0.036), but not exceeding 0.01 μm. Both GI and GII showed significant structural changes by using scanning electron microscopy with energy dispersive X-ray spectroscopy.ConclusionCarnoy's solution altered the organic and inorganic matrix of the human calcified specimens analyzed in vitro, and its effect was more pronounced when chloroform was present.



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