Evaluation of the diagnostic value of immunoglobulin clonal gene rearrangements in patients with parotid gland MALT lymphoma using BIOMED-2 protocol

Publication date: Available online 21 March 2018
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Yamin Zhang, Dan Yu, Ke Huang, Chongshang Huang, Hangfei Liu, Xiafei Sun, Jiaxiong Wang, Huiyong Zhu
ObjectivesTo evaluate the diagnostic value of immunoglobulin (Ig) clonal gene rearrangements for mucosa-associated lymphoid tissue (MALT) lymphoma of the parotid gland.MethodsWe collected and retrospectively analyzed clinical data of 21 patients referred to our institution between 2009 and 2017. Eight patients were primarily diagnosed MALT lymphoma of the parotid gland, the remaining patients diagnosed lymphoepithelial lesion (LEL). The paraffin-embedded tissues were chosen for extracting genomic DNA and multiplex primer PCR amplification using BIOMED-2 primers. PCR amplification products were analyzed by heteroduplex analysis (HA).ResultsGenerally, seventeen patients were identified to be parotid gland MALT lymphoma; 47.06% of them had Sjögren's syndrome (SS). The sensitivity of IGH VH-JH FR1, FR2, FR3, IGK Vκ-Jκ and IGK (Vκ-Kde and intron-Kde) as targets was 76.47%, 82.35%, 88.24%, 29.41% and 35.29% respectively. The sensitivity of combined application of the above three IGH primers as targets was 100%. The sensitivity of combined application of the above two IGK primers as targets was 58.82%.ConclusionIg clonal gene rearrangements assays using BIOMED-2 protocol, could be a highly-reliable diagnostic method for parotid gland MALT lymphoma. For patients with SS, along with histologically benign lymphoepithelial lesion (BLL), Ig clonal gene rearrangements is important for routine differential diagnosis.



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