Publication date: Available online 21 March 2018
Source:Archives of Oral Biology
Author(s): Jung-Sun Moon, Min-Ju Kim, Hyun-Mi Ko, Young-Jun Kim, Ji-Yeon Jung, Jae-Hyung Kim, Sun-Hun Kim, Min-Seok Kim
ObjectiveIt has been well known that Hedgehog (Hh) signaling plays an important role in bone development, however, its function in cementogenesis has not yet been reported. This study was intended to elucidate the role of Hh signaling in cementoblast differentiation.DesignExpression changes of various Hh signaling components and levels of skeletogenic markers (alkaline phosphatase, osteocalcin, osteopontin) and osteogenic transcription factors (RUNX2, Osterix) by Hh signaling modulators during OCCM-30 cementoblast differentiation were determined by quantitative real-time reverse transcriptase polymerase chain reaction. To investigate effects of Hh signaling modulators on the mineralization of cementoblast, alkaline phosphatase and alizarin red S staining were used. Then, the interaction between Hh and BMP signaling during cementoblast differentiation was evaluated using co-treatment of BMP7 and Hh signaling modulators.ResultsWe observed the consistent expression of Hh signaling molecules in the OCCM-30, which were up-regulated during cementoblast differentiation. We also found that the treatment of cells with Purmo, an Hh activator, enhanced cementoblast differentiation by increasing the mRNA expression of skeletogenic markers and osteogenic transcription factors, as well as increasing alkaline phosphate activity and mineralization capability. On the contrary, an Hh antagonist, like Cyclo, effectively inhibited cementoblast differentiation. Furthermore, BMP7 promoted cementoblast differentiation through crosstalk with the Hh signaling.ConclusionThese results suggest that Hh signaling is involved in cementoblast differentiation, and Hh signaling molecules may therefore represent new therapeutic targets in periodontal treatment and regeneration.
http://ift.tt/2pwgx1o
Αρχειοθήκη ιστολογίου
-
►
2023
(256)
- ► Φεβρουαρίου (140)
- ► Ιανουαρίου (116)
-
►
2022
(1695)
- ► Δεκεμβρίου (78)
- ► Σεπτεμβρίου (142)
- ► Φεβρουαρίου (155)
-
►
2021
(5507)
- ► Δεκεμβρίου (139)
- ► Σεπτεμβρίου (333)
- ► Φεβρουαρίου (628)
-
►
2020
(1810)
- ► Δεκεμβρίου (544)
- ► Σεπτεμβρίου (32)
- ► Φεβρουαρίου (28)
-
►
2019
(7684)
- ► Δεκεμβρίου (18)
- ► Σεπτεμβρίου (53)
- ► Φεβρουαρίου (2841)
- ► Ιανουαρίου (2803)
-
▼
2018
(31838)
- ► Δεκεμβρίου (2810)
- ► Σεπτεμβρίου (2870)
-
▼
Μαρτίου
(2963)
-
▼
Μαρ 22
(164)
- Local Anesthetic Injection Speed and Common Perone...
- Case of a fractured human bone fragment as an endo...
- What does vanishing bone disease look like?
- Klippel-Trenaunay-Weber syndrome as a cause of chr...
- Testicular torsion in a patient with Ehlers-Danlos...
- Two cases of hypokalaemic rhabdomyolysis: same but...
- A rare presentation of haematuria: hip prosthesis ...
- Importance of source images of time-of-flight magn...
- Rhinofacialentomophthoromycosis
- Central congenital hypothyroidism caused by matern...
- Tarsal buckle with conjunctival prolapse following...
- Complication of intraprosthetic dislocation of dua...
- Myocarditis secondary to smallpox vaccination
- Parkinsons disease with hypocalcaemia: adult prese...
- Diffusion-weighted imaging is helpful in the accur...
- Editorial Board
- Training Groups
- Effect of Splenectomy on Serum Cytokine Profiles i...
- A brave new world
- Intracell Heat Shock Protein 70 Expression and Nas...
- Post Parotidectomy Quality of Life in Patients wit...
- The Journal of Dermatology, Ahead of Print.
- The Journal of Dermatology, Ahead of Print.
- Intracell Heat Shock Protein 70 Expression and Nas...
- Post Parotidectomy Quality of Life in Patients wit...
- Biologicals in der Rhinologie – Individualisierte ...
- Molekulares Verstehen des Hörens – Was ändert sich...
- Das Mikrobiom – die unplanbare Größe zukünftiger T...
- HPV – Das andere Kopf-Hals-Karzinom
- Radiomics: Big Data statt Biopsie in der Zukunft?
- Robotische Chirurgie – operiert der Roboter?
- Regeneration – eine neue therapeutische Dimension ...
- Editorial Referateband 2018
- Immuntherapie – Die neue Ära in der Onkologie
- Safety of a two-day ultra-rush immunotherapy in ve...
- From the pages of allergywatch recent advances in ...
- Head &Neck, Ahead of Print.
- Head &Neck, Ahead of Print.
- Head &Neck, Ahead of Print.
- Head &Neck, Ahead of Print.
- Head &Neck, Ahead of Print.
- Thyroid® High-Impact Articles
- Discrepancy between clinical and pathological neck...
- Impact of Total Laryngectomy on Return to Work
- Salivary Pepsin Test: Useful and Simple Tool for t...
- Radioguided Surgery in Primary Hyperparathyroidism...
- Correlation Between Measures of Perceptual Assessm...
- Cleft Lip and Palate Subjects Prevalence of Abnorm...
- Efficacy of the Myofascial Pectoralis Major Flap i...
- Cochleotoxicity monitoring protocol
- Recurrent Respiratory Papillomatosis With Lung and...
- Metastasis of Gallbladder Adenocarcinoma to the Sk...
- Castleman's Disease in the Paediatric Neck
- Arteria lusoria: A rare cause of tracheal compression
- Plummer Vinson syndrome
- Comments on the Assessment of Nasal Obstruction Wi...
- A 10 Point Plan to Demonstrate the Value of Dermat...
- Functional outcomes assessment following free musc...
- Is the 8th edition of the Union for International ...
- Reply to DR. De Cassai et al.
- Is the 8th edition of the Union for International ...
- Australasian Journal of Dermatology, Ahead of Print.
- Asthma Guidelines from the National Asthma Educati...
- Ataxia Telangiectasia: A Rare Neurodegenerative Di...
- Ataxia-Telangiectasia Clinical and Laboratory Feat...
- 2017 Acknowledgment of Reviewers
- Evaluation of the diagnostic value of immunoglobul...
- The severity of denture stomatitis as related to r...
- Alterations in the expression of DNA damage respon...
- Dr. Herbert H. Frommer, 1933-2018
- Australasian Journal of Dermatology, Ahead of Print.
- Procedures for central auditory processing screeni...
- Separation Patterns of Orbital Wall and Risk of Op...
- Modified Le Fort III osteotomy: A simple solution ...
- Predicting complications of major head and neck on...
- Assessing oral health-related quality of life in c...
- Asthma Guidelines from the National Asthma Educati...
- Ataxia Telangiectasia: A Rare Neurodegenerative Di...
- Ataxia-Telangiectasia Clinical and Laboratory Feat...
- 2017 Acknowledgment of Reviewers
- What parents are reading about laryngomalacia: Qua...
- Auditory function and motor proficiency in type 1 ...
- Nasal saline irrigation in pediatric rhinosinusiti...
- Development and validation of a quality of life in...
- A functional and anatomical comparison between two...
- Three-dimensional development of the upper dental ...
- Diagnosis and management of pediatric sinusitis: A...
- Effect of early correction of nasal septal deformi...
- Obstructive sleep apnea syndrome and growth failure
- Open resection and laryngotracheal reconstruction ...
- Surgical treatment for chronic cervical lymphadeni...
- Early experience with high-flow nasal oxygen thera...
- Systematic review/meta-analysis comparing successf...
- Trends in the management of secondary post-tonsill...
- Autosomal dominant stapes fixation, syndactyly, an...
- Swallowing outcomes in children after slide trache...
- Primary augmentation pharyngoplasty with calcium h...
- Nasal erosion as an uncommon sign of child abuse
- Clinically recorded cortical auditory evoked poten...
- Pediatric Otolaryngology: Practical Clinical Manag...
-
▼
Μαρ 22
(164)
- ► Φεβρουαρίου (2420)
- ► Ιανουαρίου (2395)
-
►
2017
(31987)
- ► Δεκεμβρίου (2460)
- ► Σεπτεμβρίου (2605)
- ► Φεβρουαρίου (2785)
- ► Ιανουαρίου (2830)
-
►
2016
(5308)
- ► Δεκεμβρίου (2118)
- ► Σεπτεμβρίου (877)
- ► Φεβρουαρίου (41)
- ► Ιανουαρίου (39)
Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου