Αρχειοθήκη ιστολογίου

Αλέξανδρος Γ. Σφακιανάκης
ΩτοΡινοΛαρυγγολόγος
Αναπαύσεως 5
Άγιος Νικόλαος Κρήτη 72100
2841026182
6032607174

Παρασκευή 17 Αυγούστου 2018

Effects of hyperleptinemia in rat saliva composition, histology and ultrastructure of the major salivary glands

Publication date: Available online 17 August 2018

Source: Archives of Oral Biology

Author(s): Elsa Lamy, Sandra Neves, Joana Ferreira, Lénia Rodrigues, Gonçalo da Costa, Carlos Cordeiro, Luísa Fialho, Mónica Lima, Ana Rodrigues Costa, Célia Miguel Antunes, Orlando Lopes, Francisco Amado, Fernando Capela e Silva

Abstract
Objective

To study the effect of the satiety hormone, leptin, in saliva proteome and salivary gland histology and ultrastructure.

Design

Increases in blood leptin levels were induced through mini-pump infusion in male Wistar rats, during a period of 7 days. Saliva was collected before and at the end of the experimental period, for proteomic analysis, and major salivary glands were collected, at the end, for biochemical, histological and ultrastructural analysis.

Results

Immunohistochemistry revealed the presence of leptin receptors in major salivary glands. Salivary amylase levels and enzymatic activity were decreased in saliva, whereas the enzymatic activity of this protein was increased in the cytosol of parotid gland cells. Transmission electron microscopy allowed the observation of high number of electron-dense granules in cytosol of parotid acinar cells.

Conclusions

Increased levels of plasmatic leptin result in changes in saliva composition and salivary glands function. To our knowledge, this is the first study providing evidences for a potential role of leptin in salivary gland secretion and saliva composition. An understanding of how appetite/satiety factors influence saliva composition and how this composition influences food processing in mouth may be relevant in understanding ingestive behaviour.



https://ift.tt/2Mpq2Ot

Δεν υπάρχουν σχόλια:

Δημοσίευση σχολίου