Evaluation of HLA-G, HLA-E and PD-L1 proteins in oral osteosarcomas

Publication date: Available online 14 December 2016
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Diego Antonio Costa Arantes, Andréia Souza Gonçalves, Bruno Correia Jham, Eliza Carla Barroso Duarte, Élbio Candido de Paula, Henrique Moura de Paula, Elismauro Francisco Mendonça, Aline Carvalho Batista
ObjectiveThe aim of this study was to investigate the expression of human leukocyte antigen-G and E (HLA-G and HLA-E) and programmed death-1-ligand 1 (PD-L1) in oral osteosarcomas (OO) (n= 13). The relationship between the expression of these molecules and histological grading and metastases was also evaluated.Study DesignHLA-G, -E and PD-L1 molecules were identified by immunohistochemistry. Samples of normal bone tissues (n= 6) were used as controls. The sections were evaluated using a semi-quantitative scoring system, through an immunoreactive score (IRS), where IRS = 0 was considered absent; ≤ 2, low; and > 2 high expression of HLAs and PD-L1.ResultsWe identified high expression of HLA-G, -E and PD-L1 by malignant osteoblastic cells in 69.2% of OO cases, which was statistically higher than controls (p < 0.05). The overexpression of these proteins was identified in 8/ 11 samples of high grade and in 1/ 2 samples of low grade OO. Additionally, 66.6% (n=4) of patients with metastases, and 71.4% (n=5) of those without metastases, showed high expression of HLA-G, -E and PD-L1 in tumor samples (p > 0.05).ConclusionOO showed high expression of HLA-G, -E and PD-L1 irrespective of clinicopathological parameters, including histological grading and metastases.



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