Source:Journal of Oral and Maxillofacial Surgery
Author(s): Bin Xuan, Pan Yang, Shichao Wu, Lin Li, Jian Zhang, Wenyi Zhang
PurposeDue to the different embryological origins of the craniofacial skeleton and ilium, differences in gene expression patterns have been observed between the jaw bones and ilium. Distal-less homeobox genes and Msh homeobox genes, particularly Dlx-5 and Msx-1, play major roles in cell differentiation and osteogenesis. The purpose of this study was to investigate the effects of zoledronate on the craniofacial skeleton and ilium by detecting changes in Dlx-5 and Msx-1 expression at both the protein and mRNA levels.Materials and methodsA total of 24 female Sprague-Dawley rats were randomly divided into two groups: a zoledronate group (n=12), in which the rats were injected intraperitoneally with zoledronic acid for 12 weeks; and a control group (n=12), in which the rats were injected with saline solution for 12 weeks. Using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction (RT-PCR), the expression levels of Dlx-5 and Msx-1 in the craniofacial skeleton (including the maxilla, mandible and parietal bone) and ilium were examined.ResultsDlx-5 expression in the maxilla and mandible was increased at the protein and mRNA levels in the ZOL group compared with the control group (P<0.01). In addition, Msx-1 expression in the maxilla and mandible was decreased in the ZOL group (P<0.01). Further, Dlx-5 and Msx-1 expression in the ilium was decreased in the ZOL group (P<0.05). However, no significant difference in Dlx-5 or Msx-1 expression in the parietal bone was observed between the two groups (P>0.05).ConclusionsSite-specific differences in the effects of zoledronate on the craniofacial skeleton and ilium could be explained by differently altered tendencies in Msx-1 and Dlx-5 expression. The jaw bones were more susceptible to the effects of zoledronate than the parietal bone and ilium.
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