Regeneration of gingival tissue using in-site tissue engineering with pH 3 or pH 7.4 collagen scaffold

Publication date: Available online 11 May 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Takahide Hatayama, Akira Nakada, Hiroki Nakamura, Wakatsuki Mariko, Gentarou Tsujimoto, Tatsuo Nakamura
ObjectiveTo evaluate two types of collagen scaffold for gingival regeneration.Study designTwo types of collagen scaffolds, CS-pH7.4 and CS-pH3.0, were prepared by processing atelocollagen at pH 7.4 or 3.0, respectively, followed by dehydrothermal treatment. Gingival wounds with sizes of 4 × 6 mm (rectangle) or 6 mm diameter (circle) were made by buccal incisions in beagle dogs. The defective area was surgically covered with the CS-pH7.4, CS-pH3.0, or no scaffold (control). Gingival regeneration was assessed by monitoring the differences in the lengths of the epithelial and submucosal tissues at the wound site and -normal site. Histopathological assessments were performed by four evaluators independently; statistical significance was evaluated by the Wald test.ResultsSignificantly higher recovery of epithelial and submucosal tissues, resulting in recovery of gum thickness, was observed in gingival wounds treated with the CS-pH7.4 than that in the control. CS-pH3.0 treatment also resulted in higher gingival regeneration than the control; however, the effects were more pronounced in wounds treated with the CS-pH7.4. CS-pH7.4-treated wounds showed better gingival regeneration than control and CS-pH3.0-treated wounds, even after adjusting for interevaluator differences using a linear mixed model.ConclusionsCS-pH7.4 was a promising scaffold for gingival tissue regeneration.



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