Upregulation of eIF3a promotes cell survival in ameloblastoma

Publication date: Available online 22 February 2019

Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Author(s): Zhenjiang Ding, Jie Liu, Junting Wang, Biying Huang, Ming Zhong

Abstract

Objectives

This study aimed to detect the expression of eIF3a in ameloblastoma (AB) tissues compared with normal oral mucosa (NOM) tissues and investigate the roles of eIF3a in AM-1 cell proliferation and apoptosis.

Study Design

We performed immunohistochemistry to determine the expression of eIF3a in AB tissues (n = 83) and NOM tissues (n = 20). RT-qPCR and western blotting analyses were conducted with AB tissues (n = 30) and NOM tissues (n = 6). The correlation between eIF3a expression and the clinical/pathological features of AB patients is also presented. The functional role of eIF3a in AM-1 cells was assessed with lentiviral vector-mediated shRNA.

Results

Our results indicated that eIF3a was significantly upregulated in AB. Additionally, eIF3a knockdown in AM-1 cells significantly inhibited cell proliferation and promoted apoptosis.

Conclusion

These data indicate that eIF3a facilitates the survival of AB cells and may serve as a promising therapeutic target for AB.

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