Integrative analysis of mRNA and miRNA expression profiles in oral lichen planus: preliminary results

Publication date: Available online 8 June 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Junjun Chen, Guanhuan Du, Yufeng Wang, Linjun Shi, Jun Mi, Guoyao Tang
ObjectiveOral lichen planus (OLP) isa chronic inflammatory disease of unknown etiology that is considered an Oral Potentially Malignant Disorders. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HC).DesignBiopsies of the oral mucosa were collected from patients with OLP (n=9) and HC (n=4). Differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) were screened by next generation sequencing with DESeq and edgeR software algorithm.ResultsA total of 94 DEMs and 599 DEGs were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNA-mRNA networks. Of these, five downregulated miRNAs: hsa-miR-135a-5p (P=0.33), hsa-miR-128-3p (P=0.03), hsa-miR-218-5p (P=0.01), hsa-miR-125a-5p (P=0.01), and hsa-let-7e-5p (P=0.04) were the most promising biomarkers in OLP compared to HC. The identified DEGs were significantly enriched in 'inflammatory' events and immune-related terms through KEGG and GO analysis.ConclusionsThe integrative analysis of mRNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.



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