Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Huanzhong Ji, Jiayang Li, Jun Shao, Dongming He, Yao Liu, Wei Fei, En Luo
PurposeThe objectives of this study were to investigate the application and differential diagnostic value of safranin O staining, safranin-fast green staining and Runt-related transcription factor 2 (Runx2) immunohistochemistry in condylar hyperplasia and condylar osteochondroma.Study DesignHistopathological presence was evaluated using hematoxylin-and-eosin (HE) staining, safranin O staining, safranin-fast green staining and immunohistochemistry of Runx2 in postoperative specimens of normal condyle (control), condylar hyperplasia and condylar osteochondroma.ResultsSafranin O staining clearly highlighted the tissue structure of the condylar cartilage, especially the hypertrophic layer. The safranin-fast green method showed a contrast in staining between the cartilage and the subchondral cancellous bone in the condyle specimens. Both methods were better than HE for morphologically distinguishing condylar hyperplasia and condylar osteochondroma. The expression of Runx2 in condylar hyperplasia was significantly greater than that in condylar osteochondroma.ConclusionsThis study indicated that safranin O staining and safranin-fast green staining are effective staining methods to differentiate between condylar hyperplasia and condylar osteochondroma. Immunohistochemistry findings suggested that Runx2 is valuable in the differential diagnosis of these 2 diseases.
http://ift.tt/2fEcIlE
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου