We present the access to [5‐19F, 5‐13C] uridine and ‐cytidine phosphoramidites for the production of site‐specifically modified RNAs up to 65 nucleotides (nts). The amidites were used to introduce [5‐19F, 5‐13C]‐pyrimidine labels into five RNAs – the 30nt human immunodeficiency virus trans activation response (HIV TAR) 2 RNA, the 61nt human hepatitis B virus ε (hHBV ε) RNA, the 49nt SAM VI riboswitch aptamer domain from B. angulatum, the 29nt apical stem loop of the pre‐microRNA (miR) 21 and the...
Thu Jun 18, 2020 17:09
Inositol hexakisphosphate (InsP6) is a central member of the inositol phosphate messengers in eukaryotic cells. Tools to manipulate the level of InsP6, particularly with compartment selectivity, are needed to enable functional cellular studies. Here, we present the cationic oligoproline Z8 for the delivery of InsP6 into the cell nucleus. CD‐spectroscopic and binding affinity studies revealed that Z8 binds tightly to InsP6 and undergoes upon binding a conformational change from a PPII‐helical to a...
Thu Jun 18, 2020 17:09
An efficient Cp*Co(III)‐catalyzed C‐8 dienylation of quinoline‐ N ‐oxides was achieved employing allenes bearing leaving groups at the α ‐position as the dienylating agents. The reaction proceeds via a Co(III)‐catalyzed C‐H activation of quinoline‐ N ‐oxides, regioselective migratory insertion of allene followed by β ‐oxy elimination leading to overall dienylation. Site‐selective C‐H activation was achieved with excellent selectivity under mild conditions and inquisitively 30 mol% of NaF...
Thu Jun 18, 2020 14:41
Endocytic pathways are practical routes for the intracellular delivery of biomacromolecules. Along with this, effective strategies for endosomal cargo release into cytosol are desired to achieve successful delivery. Focused on compositional differences between the cell and endosomal membranes and the pH decrease within endosomes, we designed the lipid‐sensitive and pH‐responsive endosome lytic peptide HAad. This peptide contains aminoadipic acid (Aad) residues, which serve as a safety‐catch for preferential...
Thu Jun 18, 2020 14:37
Zeolite Y with high SiO 2 /Al 2 O 3 ratio (SAR) plays an important role in fluidized catalytic cracking process. However, in situ synthesis of zeolite Y with high SAR remains challenges due to kinetic limitations. Herein, zeolite Y with SAR of 6.35 was first synthesized via a hydroxyl radical‐assisted route coupled with post‐synthesis treatment, where hydrogen peroxide was used as the initiator of hydroxyl radicals. Density functional theory (DFT) calculations suggested that hydroxyl radicals...
Thu Jun 18, 2020 13:59
An organocatalytic enantioconvergent synthesis of chiral tetrasubstituted allenes is disclosed. With suitable chiral phosphoric acid catalysts, a range of racemic indole‐substituted propargylic alcohols reacted with nucleophiles to provide efficient access to a series of enantioenriched allenes with high enantioselectivities. Control experiments suggested a mechanism involving remotely controlled asymmetric 1,8‐addition of the in situ‐generated indole imine methide via a bifunctional transition state.
Thu Jun 18, 2020 13:48
A quasi‐pure C H polymorph of microporous zeolite beta, named ECNU‐36, was obtained as a highly crystalline silicate using 1,5‐bis(tetramethylimidazolium) hydroxide as organic structure‐directing agent (OSDA) in fluoride media. An appropriate concentration of free fluoride in the synthetic mother liquor was crucial to purify the C H ‐phase. The framework structure of ECNU‐36 consists of polymorph C H (> 95%) and polymorph B, elucidated by a combination of PXRD data, DIFFaX simulation,...
Thu Jun 18, 2020 12:29
Abstract Background Atopic eczema (atopic dermatitis, AD) is characterized by disrupted skin barrier associated with elevated skin pH and skin microbiome dysbiosis, due to high Staphylococcus aureus loads, especially during flares. Since S. aureus shows optimal growth at neutral pH, we investigated the longitudinal interplay between these factors and AD severity in a pilot study. Method Emollient (with either basic pH 8.5 or pH 5.5) was applied double blinded twice daily to 6 AD patients...
Sat Jun 20, 2020 11:36
Abstract Background Coronavirus disease 2019 (COVID‐19) emerged in Wuhan city and rapidly spread globally outside China. We aimed to investigate the role of peripheral blood eosinophil (EOS) as a marker in the course of the virus infection to improve the efficiency of diagnosis and evaluation of COVID‐19 patients. Methods 227 pneumonia patients who visited the fever clinics in Shanghai General Hospital and 97 hospitalized COVID‐19 patients admitted to Shanghai Public Health Clinical Center...
Sat Jun 20, 2020 11:35
ABSTRACT Adolescent and young adult (AYA) patients need additional support while they experience the challenges associated with their age. They need specific training to learn the knowledge and skills required to confidently self‐manage their allergies and/or asthma. Transitional care is a complex process which should address the psychological, medical, educational and vocational needs of AYA in the developmentally appropriate way. The European Academy of Allergy and Clinical Immunology has developed...
Fri Jun 19, 2020 18:31
Abstract Parabens are para‐hydroxybenzoic acid esters that have been used for almost 100 years as preservatives in cosmetics, drugs and foods.1 Sensitization to parabens was first described in the 1940s, and these compounds were subsequently included in the standard patch test batteries. Data based on weak scientific evidence have attributed parabens with carcinogenic, infertility and endocrine disruption effects. Genuine phobia towards these compounds, adoption of “paraben‐free” labeling on cosmetic...
Fri Jun 19, 2020 17:03
Objective Genetic variants of the cytoplasmic FMR1‐interacting protein 2 (CYFIP2 ) encoding an actin‐regulatory protein are associated with brain disorders, including intellectual disability and epilepsy. However, specific in vivo neuronal defects and potential treatments for CYFIP2 ‐associated brain disorders remain largely unknown. Here, we characterized Cyfip2 heterozygous (Cyfip2 +/− ) mice to understand their neurobehavioral phenotypes and underlying pathological mechanisms. Furthermore,...
Sat Jun 20, 2020 12:50
Objective To examine associations of molecular markers of brain insulin signaling with Alzheimer's disease (AD) and cognition, among older persons with or without diabetes. Methods This clinical‐pathologic study was derived from a community‐based cohort study, the Religious Orders Study. We studied 150 individuals (mean age‐at‐death =87 years, 48% women): 75 with and 75 without diabetes (matched by sex on age‐at‐death and education). Using ELISA, immunohistochemistry and ex vivo stimulation...
Thu Jun 18, 2020 18:20
OBJECTIVE To explore the role of alpha‐synuclein (αSyn) oligomers and neurofilament light chain (NFL) in cerebrospinal fluid (CSF) as markers of early multiple system atrophy (MSA) and to contrast findings to Lewy body synucleinopathies. METHODS In a discovery cohort of well‐characterized early MSA patients (n = 24) and matched healthy controls (CON, n = 14) we utilized ELISA to measure NFL and protein misfolding cyclic amplification (PMCA) to detect αSyn oligomers in CSF. We confirmed findings...
Thu Jun 18, 2020 12:10
Abstract Objectives To determine the ultrastructural changes of titanium surfaces of dental implants induced by the tip of periodontal probes. Materials and methods A total of 40 samples of smooth and rough surfaces of titanium implants were randomly assigned for the treatment with metal or plastic periodontal probes under application angles of 20° and 60°....
Sun Jun 21, 2020 03:00
Abstract Objectives To compare the healing of suprabony defects following treatment with either open flap debridement (OFD) and application of an enamel matrix derivative (EMD) with OFD alone. Methods Eighty patients with suprabony periodontal defects were randomly assigned to treatment with OFD + EMD (test) or OFD alone (control). The primary outcome variable...
Fri Jun 19, 2020 03:00
Abstract Objective The aim of this study is to investigate the efficacy of delivering low-level laser therapy (LLLT) in the management of dry socket at University Dental Hospital Sharjah. Materials and method Forty-five patients with dry socket were divided into two treatment groups. Group I dry socket patients (n = 30) received conventional treatment while...
Fri Jun 19, 2020 03:00
Abstract Objectives The separated root canal instruments may affect the quality of root canal filling, hence the success of endodontic treatment. The aim of this study was to evaluate the effects of separated file fragments of nickel-titanium rotary systems with different cross-section, taper and motion characteristics on the apical sealer penetration in oval-shaped root canals via confocal laser scanning microscope (CLSM). ...
Fri Jun 19, 2020 03:00
Aims. To confirm the therapeutic efficacy of conbercept for the treatment of macular edema (ME) secondary to retinal vein occlusion (RVO) by using optical coherence tomography angiography (OCTA) and to find out the differences in therapeutic efficacy between ischemic and nonischemic retinal vein occlusion (iRVO or non-iRVO) after conbercept treatment. Methods. In this prospective, randomized, and comparative study, 60 unilateral eyes suffered from RVO combined with macular edema were included and...
Sat Jun 20, 2020 19:50
Purpose. To investigate the relationship between gut microbiota and liver fibrosis and establish a microbiota biomarker for detecting and staging liver fibrosis. Methods. 131 Wistar rats were used in our study, and liver fibrosis was induced by carbon tetrachloride. Stool samples were collected within 72 hours after the last administration. The V4 regions of 16S rRNA gene were amplified. The sequencing data was processed using the Quantitative Insights Into Microbial Ecology (QIIME version 1.9)....
Sat Jun 20, 2020 18:50
Persistent catecholamine-resistant shock in children causes severe morbidity and mortality. We aimed to analyze the association between hemodynamics and serum lactate at different time points and 28-day mortality in children with persistent catecholamine-resistant shock. Methods. Twenty-six children with persistent catecholamine-resistant shock were enrolled, and their hemodynamics were monitored using the pulse index continuous cardiac output. Serial cardiac index (CI), systemic vascular resistant...
Sat Jun 20, 2020 18:50
Tec kinase is an important mediator in inflammatory immune response that enhances the activity of neutrophils and macrophages. However, information on its function in lipopolysaccharide- (LPS-) induced acute kidney injury (AKI) is limited. This study is aimed at determining whether Tec kinase was a regulator in AKI. An AKI model in mice was successfully established using intraperitoneal LPS. Results showed that the serum levels of creatinine (Cr), blood urea nitrogen (BUN), and cystatin-C (Cys-C)...
Sat Jun 20, 2020 17:05
Lysosomes and acidic compartments are involved in breaking down of macromolecules, membrane recycling, and regulation of signaling pathways. Here, we analyzed the role of acidic compartments during muscle differentiation and the involvement of the Wnt/beta-catenin pathway in lysosomal function during myogenesis. Acridine orange was used to localize and quantify acidic cellular compartments in primary cultures of embryonic muscle cells from Gallus gallus. Our results show an increase in acidic compartment...
Sat Jun 20, 2020 12:35
Sat Jun 20, 2020 12:20
Background. Although Henoch-Schönlein purpura nephritis (HSPN) is characterized by glomerular deposition of aberrantly glycosylated immunoglobulin A1 (IgA1), the underlying mechanism of HSPN progression has not yet been completely elucidated. In this study, we integrated transcriptomic and proteomic analyses to explore the underlying mechanism of HSPN progression. Methods. RNA sequencing and tandem mass tag- (TMT-) based quantitative proteomics were used to gain serum transcriptomic and proteomic...
Sat Jun 20, 2020 12:05
Objective. We aimed to investigate whether apatinib has an inhibitory effect on the invasion and metastasis of liver cancer in vitro. Methods. The anti-invasion and antimetastasis effects of apatinib in HepG2, Hep3B,Huh7 and SMMC-7721 liver cancer cell lines were tested by the wound-healing and transwell invasion assays. Real-time PCR and Western blot were used to detect the influence of apatinib on the gene expression of MMPs, TIMPs, and constituents of the NF-κB signaling pathway in Hep3B and HepG2...
Sat Jun 20, 2020 09:05
Background. The thyroid hormone metabolite 3-iodothyronamine (3-T1AM) is rapidly emerging as a promising compound in decreasing the heart rate and lowering the cardiac output. The aim of our study was to fully understand the molecular mechanism of 3-T1AM on cardiomyocytes and its potential targets in cardiovascular diseases. Materials and Methods. In our study, we utilized RNA-Seq to characterize the gene expression in H9C2 cells after 3-T1AM treatment. Comparative transcriptome analysis, including...
Sat Jun 20, 2020 06:05
Background. To investigate the effect of hemodialysis on microbiota in uremic patients. Objective. To investigate the effect of hemodialysis on microbiota in uremic patients. Methods. This study included 85 adult patients who have received hemodialysis since August 2014, and the treatment plan has not changed for more than 12 months. These patients were divided into hemodialysis group (group A), hemodialysis+hemodialysis filtration group (group B), and hemodialysis+hemodialysis filtration+blood perfusion...
Fri Jun 19, 2020 16:50
The protein 14-3-3δ interacts with Trp53 to maintain G2 arrest and thus regulates the cell cycle. Though dysfunction of 14-3-3δ caused by hyper-methylation of CpG islands was reported in several carcinomas, the exact role of this protein in the development of extrahepatic cholangiocarcinoma has not been fully elucidated. Here, we aim at investigating the clinical relevance between 14-3-3δ and human extrahepatic cholangiocarcinoma. We collected extrahepatic cholangiocarcinoma specimens of 65 patients...
Fri Jun 19, 2020 10:50
Background. Worldwide lack of sanitation is a serious health risk, affecting billions of people around the world, particularly the poor and disadvantaged of people around the world. In Sub-Saharan Africa, the number of people who defecate remains the open field 215 million. According to the 2016 Ethiopian Demographic and Health Surveys report, 56% of the rural households use unimproved toilet facilities. One in every three households in the country has no toilet facility. However, achieving real...
Fri Jun 19, 2020 09:05
Purslane, a fleshy herbaceous plant, plays a pivotal role in various preventive and therapeutic purposes. To date, no report has documented the consequence of salt stress on metabolite accumulation in purslane. Herein, we proposed an insight into the metabolic and physiological traits of purslane under saline stress environments. The gas chromatography-mass spectrometry analysis was used to scrutinize the metabolic profiling of leaves and roots of two purslane genotypes, Tall Green (TG) and Shandong...
Fri Jun 19, 2020 08:35
Hepatitis B virus is a widespread public health menace approximated to have infected 257 million people chronically by 2015. Data on the prevalence of HBV is important in formulating public health policies on HBV control like safe blood transfusion. Adolescents aged 15 to 24 years, known to engage in risky activities associated with HBV spread, constitute major blood donors in Kenya. Notwithstanding current blood donation safety measures, HBV still remain hazardous transfusion-transmissible infections...
Fri Jun 19, 2020 07:50
Chemical fertilizers were applied on perennial tree vines to obtain high yields, which have resulted in considerable deterioration of soil quality, and it is likely to have negative impacts on the development of the grape industry. In this study, P. putida Rs-198 liquid biofertilizer (Rs198LBF) was inoculated into grape rhizosphere soils to assess its influence on grape growth and microbial community. Field experiment results showed that grape growth and quality increased depending on the concentrations...
Fri Jun 19, 2020 07:50
Summary Purpose Part A of the open-label, phase I KEYNOTE-434 study evaluated the safety and tolerability of epacadostat, an indoleamine 2,3-dioxygenase-1 inhibitor, alone and in combination with pembrolizumab in Japanese patients with advanced solid tumors. Methods Japanese patients with refractory/recurrent metastatic or locally advanced tumors were...
Sat Jun 20, 2020 03:00
To investigate the mechanism dexmedetomidine in relieving the neurotoxicity of a developing brain induced by sevoflurane. Sprague-Dawley rats, 6 days old, were randomly divided into three groups. Rats in the control group were inhaled with air after injection of normal saline; rats in the sevoflurane group were injected with normal saline and inhaled with 3% sevoflurane for 2 h in three consecutive day; rats in the dexmedetomidine group were inhaled with 3% sevoflurane after intraperitoneal injection...
Sat Jun 20, 2020 14:20
Postoperative cognitive dysfunction (POCD) is a common complication after surgery, especially in aged patients. Neuroinflammation has been closely associated with the development of POCD. While the contribution of pneumoperitoneum to the systemic inflammation has been well documented, the effect of pneumoperitoneal pressure on neuroinflammation and postoperative cognitive function remains unclear. In this study, we showed that high-pressure pneumoperitoneum promoted the postoperative neuroinflammation...
Fri Jun 19, 2020 17:50
Abstract The main goal of the present study was estimation of an internal contamination of 131I among family members of patients treated with radioactive iodine. Thyroid activity measurements of 131I in examined volunteers were performed using a whole-body spectrometer at the institute of nuclear physics, Polish academy of sciences. During this research, 20 relatives of patients treated with 131I were examined: eight women and 12 men with an age in the range from 3 to 72 years....
Sat Jun 20, 2020 03:00
Abstract Electron paramagnetic resonance (EPR) signals generated by ionizing radiation in touch-screen glasses have been reported as useful for personal dosimetry in people accidently exposed to ionizing radiation. This article describes the effect of light exposure on EPR spectra of various glasses obtained from mobile phones. This effect can lead to significant inaccuracy of the radiation doses reconstructed by EPR. The EPR signals from samples unexposed and exposed to X-rays...
Sat Jun 20, 2020 03:00
Abstract In radiation therapy, it is very important to ensure that the radiation dose is correctly delivered to the patient. This is achieved by obtaining quantitative dose measurements for beam calibration in the treatment planning system. Dose calculations should be performed with the required accuracy to a degree of uncertainty of less than 1%. The measurement of the absorbed dose in and around body tissues irradiated with carbon ions requires careful use of materials selected...
Fri Jun 19, 2020 03:00
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