Publication date: Available online 1 June 2020Source: NeuroImageAuthor(s): John R. Anderson, Shawn Betts, Jon M. Fincham, Ryan Hope, Mathew W. Walsh
Mon Jun 01, 2020 20:55
Publication date: Available online 1 June 2020Source: NeuroImageAuthor(s): Guillaume J.-P.C. Becq, Tarik Habet, Nora Collomb, Margaux Faucher, Chantal Delon-Martin, Véronique Coizet, Sophie Achard, Emmanuel L. Barbier
Mon Jun 01, 2020 20:55
Publication date: Available online 31 May 2020Source: NeuroImageAuthor(s): Maximilian Nentwich, Lei Ai, Jens Madsen, Qawi K. Telesford, Stefan Haufe, Michael P. Milham, Lucas C. Parra
Mon Jun 01, 2020 20:55
Publication date: Available online 31 May 2020Source: NeuroImageAuthor(s): Bo Li, Marius de Groot, Rebecca M.E. Steketee, Rozanna Meijboom, Marion Smits, Meike W. Vernooij, M. Arfan Ikram, Jiren Liu, Wiro J. Niessen, Esther E. Bron
Mon Jun 01, 2020 20:55
Visualization of Live, Mammalian Neurons During Kainate-Infusion Using Magnetic Resonance Microscopy
Publication date: Available online 31 May 2020Source: NeuroImageAuthor(s): Jeremy J. Flint, Kannan Menon, Brian Hansen, John Forder, Stephen J. Blackband
Mon Jun 01, 2020 20:55
Publication date: Available online 31 May 2020Source: Journal of Cranio-Maxillofacial SurgeryAuthor(s): Maeve Cooney, John Edward O’Connell, Jennifer Alexandra Vesey, Simon Van Eeden
Mon Jun 01, 2020 20:41
Publication date: Available online 31 May 2020Source: Gait & PostureAuthor(s): Paul W. Kline, Amanda M. Murray, Matthew J. Miller, Noel So, Thomas Fields, Cory L. Christiansen
Mon Jun 01, 2020 20:26
Perinatal exposure to omega-3 fatty acid imbalance leads to early behavioral alterations in rat pups
Publication date: Available online 31 May 2020Source: Behavioural Brain ResearchAuthor(s): Paola Colucci, Valentina De Castro, Andrea Peloso, Marta Splendori, Viviana Trezza, Patrizia Campolongo
Mon Jun 01, 2020 19:37
Publication date: Available online 31 May 2020Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): L de Gabory, A. Alharbi, M. Kérimian, M.-E. Lafon
Mon Jun 01, 2020 19:06
Publication date: Available online 31 May 2020Source: The Journal of Prosthetic DentistryAuthor(s): Daniel Alberto Cortes Trevino, Michael E. Razzoog, Berna Saglik, Diogo do Nascimento Machado Gouveia
Mon Jun 01, 2020 18:53
Publication date: Available online 31 May 2020Source: The Journal of Prosthetic DentistryAuthor(s): Oscar Figueras-Alvarez, Francisco Real-Voltas
Mon Jun 01, 2020 18:53
Publication date: Available online 31 May 2020Source: The Journal of Prosthetic DentistryAuthor(s): Marta Revilla-León, Zachary Smith, Mohammed M. Methani, Amirali Zandinejad, Mutlu Özcan
Mon Jun 01, 2020 18:53
The black yeast-like fungus Arthrocladium fulminans is known from strains that cause severe and eventually fatal disseminated infections in immunocompromised patients. Given the dramatic outcome of this clinical case, it is essential to understand the virulence potential of this species. The fungus is a member of the family Trichomeriaceae, at some phylogenetic distance from the Herpotrichiellaceae where most infectious fungi in the order Chaetothyriales are located. Main ecological preferences among...
Mon Jun 01, 2020 16:14
Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv. Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv. Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome...
Mon Jun 01, 2020 16:14
Arctic charr (Salvelinus alpinus) is a species of high economic value for the aquaculture industry, and of high ecological value due to its Holarctic distribution in both marine and freshwater environments. Novel genome sequencing approaches enable the study of population and quantitative genetic parameters even on species with limited or no prior genomic resources. Low coverage genotyping by sequencing (GBS) was applied in a selected strain of Arctic charr in Sweden originating from a landlocked...
Mon Jun 01, 2020 16:14
Woodpeckers are found in nearly every part of the world and have been important for studies of biogeography, phylogeography, and macroecology. Woodpecker hybrid zones are often studied to understand the dynamics of introgression between bird species. Notably, woodpeckers are gaining attention for their enriched levels of transposable elements (TEs) relative to most other birds. This enrichment of TEs may have substantial effects on molecular evolution. However, comparative studies of woodpecker genomes...
Mon Jun 01, 2020 16:14
G-quadruplexes are nucleic acid secondary structures formed by a stack of square planar G-quartets. G-quadruplexes were implicated in many biological functions including telomere maintenance, replication, transcription, and translation, in many species including humans and plants. For wheat, however, though it is one of the world’s most important staple food, no G-quadruplex studies have been reported to date. Here, we computationally identify putative G4 structures (G4s) in wheat genome for the...
Mon Jun 01, 2020 16:14
The first meeting of the CIFAR Fungal Kingdom: Threats & Opportunities research program saw the congregation of experts on fungal biology to address the most pressing threats fungi pose to global health, agriculture, and biodiversity. This report covers the research discussed during the meeting and the advancements made toward mitigating the devastating impact of fungi on plants, animals, and humans.
Mon Jun 01, 2020 16:14
Segregation Distorter (SD) is an autosomal meiotic drive system found worldwide in natural populations of Drosophila melanogaster. This gene complex induces the preferential and nearly exclusive transmission of the SD chromosome in SD/SD+ males. This selfish propagation occurs through the interplay of the Sd locus, its enhancers and the Rsps locus during spermatid development. The key distorter locus, Sd, encodes a truncated but enzymatically active RanGAP (RanGTPase-activating protein), a key nuclear...
Mon Jun 01, 2020 16:14
FUS is a nucleic acid binding protein that, when mutated, cause a subset of familial amyotrophic lateral sclerosis (ALS). Expression of FUS in yeast recapitulates several pathological features of the disease-causing mutant proteins, including nuclear to cytoplasmic translocation, formation of cytoplasmic inclusions, and cytotoxicity. Genetic screens using the yeast model of FUS have identified yeast genes and their corresponding human homologs suppressing FUS induced toxicity in yeast, neurons and...
Mon Jun 01, 2020 16:14
Mouse sex chromosomes are enriched for co-amplified gene families, present in tens to hundreds of copies. Co-amplification of Slx/Slxl1 on the X chromosome and Sly on the Y chromosome are involved in dose-dependent meiotic drive, however the role of other co-amplified genes remains poorly understood. Here we demonstrate that the co-amplified gene family on the X chromosome, Srsx, along with two additional partial gene annotations, is actually part of a larger transcription unit, which we name Laidx....
Mon Jun 01, 2020 16:14
Homologous recombination is an important mechanism for genome integrity maintenance, and several homologous recombination genes are mutated in various cancers and cancer-prone syndromes. However, since in some cases homologous recombination can lead to mutagenic outcomes, this pathway must be tightly regulated, and mitotic hyper-recombination is a hallmark of genomic instability. We performed two screens in Saccharomyces cerevisiae for genes that, when deleted, cause hyper-recombination between direct...
Mon Jun 01, 2020 16:14
The use of glyphosate-based herbicides is widespread and despite their extensive use, their effects are yet to be deciphered completely. The additives in commercial formulations of glyphosate, though labeled inert when used individually, have adverse effects when used in combination with other additives along with the active ingredient. As a species, Saccharomyces cerevisiae has a wide range of resistance to glyphosate-based herbicides. To investigate the underlying genetic differences between sensitive...
Mon Jun 01, 2020 16:14
Metastatic colonization, whereby a disseminated tumor cell is able to survive and proliferate at a secondary site, involves both tumor cell-intrinsic and -extrinsic factors. To identify tumor cell-extrinsic (microenvironmental) factors that regulate the ability of metastatic tumor cells to effectively colonize a tissue, we performed a genome-wide screen utilizing the experimental metastasis assay on mutant mice. Mutant and wildtype (control) mice were tail vein-dosed with murine metastatic melanoma...
Mon Jun 01, 2020 16:14
A combined multistage linear genomic selection index (CMLGSI) is a linear combination of phenotypic and genomic estimated breeding values useful for predicting the individual net genetic merit, which in turn is a linear combination of the true unobservable breeding values of the traits weighted by their respective economic values. The CMLGSI is a cost-saving strategy for improving multiple traits because the breeder does not need to measure all traits at each stage. The optimum (OCMLGSI) and decorrelated...
Mon Jun 01, 2020 16:14
Protein quality control in the cytosol (CytoQC) is an important cellular pathway consisting of a network of components which monitor the folding of cytosolic proteins and ensure the efficient removal of aberrant ones. Our understanding of CytoQC mechanisms is limited in part by the ability of current approaches to identify new genes in the pathway. In this study, we developed a CytoQC reporter substrate, Ste6*C-HA-Ura3, for a new genetic selection of spontaneous CytoQC mutations in the yeast Saccharomyces...
Mon Jun 01, 2020 16:14
We have developed a CRISPR/Cas9 based method for isolating randomly induced recessive lethal mutations in a gene of interest (GOI) by selection within the F1 progeny of a single genetic cross. Our method takes advantage of the ability to overexpress a GOI using CRISPR/Cas9 mediated activation of gene expression. In essence, the screening strategy is based upon the idea that if overexpression of a wild type allele can generate a phenotype, then overexpression of a newly induced loss-of-function allele...
Mon Jun 01, 2020 16:14
To investigate factors influencing pre-mRNA splicing in plants, we conducted a forward genetic screen using an alternatively-spliced GFP reporter gene in Arabidopsis thaliana. This effort generated a collection of sixteen mutants impaired in various splicing-related proteins, many of which had not been recovered in any prior genetic screen or implicated in splicing in plants. The factors are predicted to act at different steps of the spliceosomal cycle, snRNP biogenesis pathway, transcription, and...
Mon Jun 01, 2020 16:14
LRRK2 mutations cause Parkinson’s, but the molecular link from increased kinase activity to pathological neurodegeneration remains undetermined. Previous in vitro assays indicate that LRRK2 substrates include at least 8 Rab GTPases. We have now examined this hypothesis in vivo in a functional, electroretinogram screen, expressing each Rab with/without LRRK2-G2019S in selected Drosophila dopaminergic neurons. Our screen discriminated Rab10 from Rab3. The strongest Rab/LRRK2-G2019S interaction is with...
Mon Jun 01, 2020 16:14
Novel pathogens can cause massive declines in populations, and even extirpation of hosts. But disease can also act as a selective pressure on survivors, driving the evolution of resistance or tolerance. Bat white-nose syndrome (WNS) is a rapidly spreading wildlife disease in North America. The fungus causing the disease invades skin tissues of hibernating bats, resulting in disruption of hibernation behavior, premature energy depletion, and subsequent death. We used whole-genome sequencing to investigate...
Mon Jun 01, 2020 16:14
The R-package MoBPS provides a computationally efficient and flexible framework to simulate complex breeding programs and compare their economic and genetic impact. Simulations are performed on the base of individuals. MoBPS utilizes a highly efficient implementation with bit-wise data storage and matrix multiplications from the associated R-package miraculix allowing to handle large scale populations. Individual haplotypes are not stored but instead automatically derived based on points of recombination...
Mon Jun 01, 2020 16:14
CRISPR/Cas9 has become the preferred gene-editing technology to obtain loss-of-function mutants in plants, and hence a valuable tool to study gene function. This is mainly due to the easy reprogramming of Cas9 specificity using customizable small non-coding RNAs, and to the possibility of editing several independent genes simultaneously. Despite these advances, the identification of CRISPR-edited plants remains time and resource-intensive. Here, based on the premise that one editing event in one...
Mon Jun 01, 2020 16:14
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