Effect of nicotine on the apoptosis of odontoblasts
Author(s): GUO Xiao-juan, YUAN Jie, WANG Sheng-chao, YU Qing, QU Tie-jun, Department of Stomatology, The People's Hospital of Pingliang City
Journal: Chinese Journal of Conservative Dentistry, Year 2015 , Issue 7 , Page 400-403+399
Keyword: nicotine; MDPC-23 cell line; cell apoptosis;
Abstract: AIM: To investigate the effects of nicotine on the apoptosis of mouse odontoblasts MDPC-23.METHODS: Cultured MDPC-23 cells were treated with nicotine( μg / m L) at 0( control group),1,10 and 100 respectively. After treatment,cell morphology changes were detected by Hoechst staining,and the expression of apoptosis- related proteins of Bcl-2,Bax,Caspase-3 and p-JNK were respectively determined by Western blot. A combination of nicotine and JNK inhibitor SP600125 were also added to MDPC-23 cell culture and the expression of Caspase-3 were examined. RESULTS: With the increase of nicotine concentration(0 ~ 100 μg / m L),the cell morphology distinctly changed. The expression of Bcl-2 were downregulated and the expression of Bax,Caspase- 3 were upregulated gradually. Meanwhile,the expression of p-JNK were increased by nicotine in a dose- and time-dependent manner.Whereas,SP600125 could inhibit the nicotine- induced expresssion of Caspase- 3. CONCLUSION: Nicotine has toxic effects on MDPC-23 cells,which may promote the apoptosis of odontoblasts to some extent.
http://caod.oriprobe.com/articles/46356251/Effect_of_nicotine_on_the_apoptosis_of_odontoblast.htm
Author(s): GUO Xiao-juan, YUAN Jie, WANG Sheng-chao, YU Qing, QU Tie-jun, Department of Stomatology, The People's Hospital of Pingliang City
Journal: Chinese Journal of Conservative Dentistry, Year 2015 , Issue 7 , Page 400-403+399
Keyword: nicotine; MDPC-23 cell line; cell apoptosis;
Abstract: AIM: To investigate the effects of nicotine on the apoptosis of mouse odontoblasts MDPC-23.METHODS: Cultured MDPC-23 cells were treated with nicotine( μg / m L) at 0( control group),1,10 and 100 respectively. After treatment,cell morphology changes were detected by Hoechst staining,and the expression of apoptosis- related proteins of Bcl-2,Bax,Caspase-3 and p-JNK were respectively determined by Western blot. A combination of nicotine and JNK inhibitor SP600125 were also added to MDPC-23 cell culture and the expression of Caspase-3 were examined. RESULTS: With the increase of nicotine concentration(0 ~ 100 μg / m L),the cell morphology distinctly changed. The expression of Bcl-2 were downregulated and the expression of Bax,Caspase- 3 were upregulated gradually. Meanwhile,the expression of p-JNK were increased by nicotine in a dose- and time-dependent manner.Whereas,SP600125 could inhibit the nicotine- induced expresssion of Caspase- 3. CONCLUSION: Nicotine has toxic effects on MDPC-23 cells,which may promote the apoptosis of odontoblasts to some extent.
http://caod.oriprobe.com/articles/46356251/Effect_of_nicotine_on_the_apoptosis_of_odontoblast.htm
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