Study on the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes
Author(s): WANG Junrui, WEI Changmei, TA La, CUI Jinghua, DU Xiaoli, HAN Yanqiu, Department of Clinical Laboratory, the Affiliated Hospital of Inner Mongolia Medical University, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
Journal: Shanghai Journal of Medical Laboratory Sciences, Year 2015 , Issue 7 , Page 740-746
Keyword: Staphylococcus aureus; Drug resistance; In vitro invasion; Molecular typing;
Abstract: Objective To determine the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes,and further to investigate the pathogenesis mechanism of Staphylococcus aureus and provide experimental reference for controlling its nosocomial infection and transmission. Methods Pulse field gel electrophoresis( PFGE) and multilocus sequence typing( MLST) were used to do molecular typing for 43 isolates of Staphylococcus aureus,and antibiotics protection assay and flow cytometry internalization assay were used to determine the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes. Results A total of 43 isolates of Staphylococcus aureus were classified into 12 PFGE genotypes according to 80% similarity,among which type Ⅰ and type Ⅱ were the predominant types and accounted for 25. 6%( 11 /43) and 34. 9%( 15 /43),respectively. Other non-predominant types accounted for 39. 5%( 17 /43). In vitro antibiotics susceptibility test results showed that type Ⅰ and other non-predominant types were mainly composed of methicillin-sensitive Staphylococcus aureus( MSSA)( 85. 7%,24 /28),while type Ⅱ were mainly composed of methicillin-resistant Staphylococcus aureus( MRSA)( 100. 0%,15 /15). MLST revealed that type Ⅱ isolates were all ST-239 type,but type Ⅰ and other types belonged to diverse ST types. Antibiotics protection assay showed that the invasion rates of the 3 isolates with the strongest invasion abilities were 3. 27%,3. 15% and 3. 09%,respectively. The in vitro invasion abilities of type Ⅰ and type Ⅱ isolates were significantly higher than those of other non-predominant types( P < 0. 01). Conclusions Staphylococcus aureus clinical isolates with different genotypes shows the diversity of in vitro invasion abilities,and the predominant clones revealed stronger in vitro invasion abilities,especially for ST-239 type. The units with higher Staphylococcus aureus isolation rates should pay more attention to this clone and strengthen infection control measures.
http://caod.oriprobe.com/articles/46334912/Study_on_the_diversity_of_in_vitro_invasion_abilit.htm
Author(s): WANG Junrui, WEI Changmei, TA La, CUI Jinghua, DU Xiaoli, HAN Yanqiu, Department of Clinical Laboratory, the Affiliated Hospital of Inner Mongolia Medical University, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
Journal: Shanghai Journal of Medical Laboratory Sciences, Year 2015 , Issue 7 , Page 740-746
Keyword: Staphylococcus aureus; Drug resistance; In vitro invasion; Molecular typing;
Abstract: Objective To determine the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes,and further to investigate the pathogenesis mechanism of Staphylococcus aureus and provide experimental reference for controlling its nosocomial infection and transmission. Methods Pulse field gel electrophoresis( PFGE) and multilocus sequence typing( MLST) were used to do molecular typing for 43 isolates of Staphylococcus aureus,and antibiotics protection assay and flow cytometry internalization assay were used to determine the diversity of in vitro invasion abilities of Staphylococcus aureus clinical isolates with different genotypes. Results A total of 43 isolates of Staphylococcus aureus were classified into 12 PFGE genotypes according to 80% similarity,among which type Ⅰ and type Ⅱ were the predominant types and accounted for 25. 6%( 11 /43) and 34. 9%( 15 /43),respectively. Other non-predominant types accounted for 39. 5%( 17 /43). In vitro antibiotics susceptibility test results showed that type Ⅰ and other non-predominant types were mainly composed of methicillin-sensitive Staphylococcus aureus( MSSA)( 85. 7%,24 /28),while type Ⅱ were mainly composed of methicillin-resistant Staphylococcus aureus( MRSA)( 100. 0%,15 /15). MLST revealed that type Ⅱ isolates were all ST-239 type,but type Ⅰ and other types belonged to diverse ST types. Antibiotics protection assay showed that the invasion rates of the 3 isolates with the strongest invasion abilities were 3. 27%,3. 15% and 3. 09%,respectively. The in vitro invasion abilities of type Ⅰ and type Ⅱ isolates were significantly higher than those of other non-predominant types( P < 0. 01). Conclusions Staphylococcus aureus clinical isolates with different genotypes shows the diversity of in vitro invasion abilities,and the predominant clones revealed stronger in vitro invasion abilities,especially for ST-239 type. The units with higher Staphylococcus aureus isolation rates should pay more attention to this clone and strengthen infection control measures.
http://caod.oriprobe.com/articles/46334912/Study_on_the_diversity_of_in_vitro_invasion_abilit.htm
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