Application of MALDI-TOF MS in direct identification of clinical microbiological samples
Author(s): CHEN Fei, HU Binjie, ZHAO Hu, Department of Clinical Laboratory, Huadong Hospital, Fudan University
Journal: Shanghai Journal of Medical Laboratory Sciences, Year 2015 , Issue 7 , Page 750-756
Keyword: Matrix-assisted laser desorption / ionization-time of flight mass spectrometry; Direct identification; Microbial identification; Clinical sample;
Abstract: Matrix-assisted laser desorption / ionization-time of flight mass spectrometry( MALDI-TOF MS),as a newly developed technology in recent years,is an easy,rapid,accurate and cost-effective method for microbial identification in clinical microbiology,compared to conventional phenotypic techniques or molecular biology. Given the accuracy and reliability of MALDI-TOF MS in the identification of microorganisms grown on solid medium,this technology might also be directly applied to some clinical microbiological samples,such as positive blood culture bottles,midstream urine,cerebrospinal fluid and so on. The goal of this review was to discuss the application of MALDI-TOF MS technology in direct identification of clinical microbiological samples,including the principles,protocols,performance and research progress.
http://caod.oriprobe.com/articles/46334903/Application_of_MALDI_TOF_MS_in_direct_identificati.htm
Author(s): CHEN Fei, HU Binjie, ZHAO Hu, Department of Clinical Laboratory, Huadong Hospital, Fudan University
Journal: Shanghai Journal of Medical Laboratory Sciences, Year 2015 , Issue 7 , Page 750-756
Keyword: Matrix-assisted laser desorption / ionization-time of flight mass spectrometry; Direct identification; Microbial identification; Clinical sample;
Abstract: Matrix-assisted laser desorption / ionization-time of flight mass spectrometry( MALDI-TOF MS),as a newly developed technology in recent years,is an easy,rapid,accurate and cost-effective method for microbial identification in clinical microbiology,compared to conventional phenotypic techniques or molecular biology. Given the accuracy and reliability of MALDI-TOF MS in the identification of microorganisms grown on solid medium,this technology might also be directly applied to some clinical microbiological samples,such as positive blood culture bottles,midstream urine,cerebrospinal fluid and so on. The goal of this review was to discuss the application of MALDI-TOF MS technology in direct identification of clinical microbiological samples,including the principles,protocols,performance and research progress.
http://caod.oriprobe.com/articles/46334903/Application_of_MALDI_TOF_MS_in_direct_identificati.htm
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου