The expression of XIAP in cerebral ischemia and research of progesterone intervention
Author(s): ZHU Zhongjun, WANG Jianping, ZHAO Yuanzheng, People's Hospital of Zhengzhou
Journal: Journal of Apoplexy and Nervous Diseases, Year 2015 , Issue 7 , Page 619-622
Keyword: Progesterone; Cerebral; Apoptosis; XIAP; Rats;
Abstract: Objective This study was designed to investigate the expression of XIAP in ischemia penumbra of cerebral infarction rats and research about the influence of progesterone on expression of XIAP and apoptosis in cerebral ischemia in rats. Methods All S-D rats,weighted 260 g ~ 310 g,were randomly divided into four groups: sham-operated( control) group,ischemic group,vehicle-treated group and PROG-treated group. Immunohistochemistry and western Western blot techniques were applied to detect the expression of XIAP at 12 h,24 h and 48 h after permanent middle cerebral artery occlusion( p MCAO),Td T-mediated d UTP nick end labeling( TUNEL) was used to investigate apoptosis cell in the brain tissues. One-way ANOVA was used to check any changes in XIAP and brain cell apoptosis after p MCAO. Results The expression of XIAP were was significantly increased in ischemia penumbra after cerebral infarction in comparison with vehicletreated groups( P < 0. 05),following PROG treatment,the expression of XIAP were was significantly increased in comparison with ischemic groups and vehicle-treated groups( P < 0. 05). In addition,PROG also significantly reduced apoptosis cells of injured brain when compared with ischemic groups and vehicle-treated groups( P < 0. 05). Conclusion PROG significantly decreases the cells apoptosis in ischemia penumbra brain,increasing the expression of XIAP may be one of the molecule mechanisms of the neuroprotective effects of PROG in injured brain.
http://caod.oriprobe.com/articles/46356448/The_expression_of_XIAP_in_cerebral_ischemia_and_re.htm
Author(s): ZHU Zhongjun, WANG Jianping, ZHAO Yuanzheng, People's Hospital of Zhengzhou
Journal: Journal of Apoplexy and Nervous Diseases, Year 2015 , Issue 7 , Page 619-622
Keyword: Progesterone; Cerebral; Apoptosis; XIAP; Rats;
Abstract: Objective This study was designed to investigate the expression of XIAP in ischemia penumbra of cerebral infarction rats and research about the influence of progesterone on expression of XIAP and apoptosis in cerebral ischemia in rats. Methods All S-D rats,weighted 260 g ~ 310 g,were randomly divided into four groups: sham-operated( control) group,ischemic group,vehicle-treated group and PROG-treated group. Immunohistochemistry and western Western blot techniques were applied to detect the expression of XIAP at 12 h,24 h and 48 h after permanent middle cerebral artery occlusion( p MCAO),Td T-mediated d UTP nick end labeling( TUNEL) was used to investigate apoptosis cell in the brain tissues. One-way ANOVA was used to check any changes in XIAP and brain cell apoptosis after p MCAO. Results The expression of XIAP were was significantly increased in ischemia penumbra after cerebral infarction in comparison with vehicletreated groups( P < 0. 05),following PROG treatment,the expression of XIAP were was significantly increased in comparison with ischemic groups and vehicle-treated groups( P < 0. 05). In addition,PROG also significantly reduced apoptosis cells of injured brain when compared with ischemic groups and vehicle-treated groups( P < 0. 05). Conclusion PROG significantly decreases the cells apoptosis in ischemia penumbra brain,increasing the expression of XIAP may be one of the molecule mechanisms of the neuroprotective effects of PROG in injured brain.
http://caod.oriprobe.com/articles/46356448/The_expression_of_XIAP_in_cerebral_ischemia_and_re.htm
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